I love this one web comic called "Questionable content". It's about a group of friends up in boston. This commic is updated every day Mon thru friday. It also has over 2000 pages. I have read the whole thing through about 13 times and I laugh my butt off each time. Here is the link.

http://www.questionablecontent.net/index.php

You can really see how the art work as debeloped over time.
There are some lesbian relation ships.
No TG but there is a tiny boy robot that sometimes crossdresses.